Asian Journal of Research in Chemistry

S. D. Jadhav ¹, M. S. Bhatia ², S. L. Thamake ², S. A. Pishawikar ²

Affiliations
1 Department of Pharmaceutical Chemistry, Tatyasaheb Kore College of Pharmacy, Warananagar-416113 (M.S.), IN
2 Department of Pharmaceutical Chemistry, Bharati Vidyapeeth College of Pharmacy, Morewadi, Kolhapur-416013 (M.S.), IN

Abstract

Lamivudine (LMV), Zidovudine (ZDV) and Nevirapine (NVR) are used in combination for treatment of HIV I. The present work deals with method development for simultaneous estimation of these drugs in tablet dosage form by UV spectrophotometry. A new simple, accurate and sensitive method for simultaneous estimation of lamivudine, zidovudine and nevirapine from tablet formulation has been developed. The method employs combination of zero order and first order derivative spectroscopy. Estimation of nevirapine was done in zero order derivative mode at 310 nm where zidovudine and lamivudine do not show any interference. Estimation of zidovudine was done in first order derivative mode at 270 nm where lamivudine and nevirapine shows zero crossing points. Estimation of lamivudine is done in zero order derivative mode at 271 nm by correcting the absorbance interferences due to zidovudine and nevirapine. Absorbance interferences of zidovudine and nevirapine calculated from their absorptivities. The results of analysis validated statistically and recovery studies showed satisfactory results. Thus the method is accurate and reproducible and can be employed for routine analysis of these drugs from tablet dosage forms.

Keywords

Lamivudine, Zidovudine, Nevirapine, Absorbance Interferences.

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S. L. Thamake ¹, S. D. Jadhav ¹, S. A. Pishawikar ¹

Affiliations
1 Department of Pharmaceutical Chemistry, Bharati Vidyapeeth College of Pharmacy, Near Chitranagari, Kolhapur-416013, IN

Abstract

Atorvastatin Calcium and Ramipril are used in combination for treatment of hypertension. The present work deals with simple spectrophotometric method development for simultaneous estimation of Atorvastatin Calcium (ATR) and Ramipril (RAM) in two component capsule formulation. The method employed first order derivative spectroscopy. For determination of sampling wavelength 10 μg/ml of each of ATR and RAM were scanned in 200-350 nm range and sampling wavelengths were 294 nm for ATR were RAM showed zero crossing point and 229 nm for RAM were ATR showed zero crossing point in first order derivative spectroscopy. For this method linearity observed in 5-40 μg/ml for ATR and 2-20 μg/ml for RAM. The recovery studies confirmed accuracy of proposed method and low values of standard deviation confirmed precision of method. The method is validated as per ICH guidelines.

Keywords

Atorvastatin Calcium, Ramipril, Derivative Spectroscopy.

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